Oligonucleotide laixin wang pdf bioanalysis

Oligonucleotides and nucleic acids interest group workshop. The bioanalysis of oligonucleotide therapeutics is constantly evolving, primarily driven by drug development needs and regulatory requirements. Find contacts direct phone number, email address, work history, and more. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Pdf bioanalysis of sirna and oligonucleotide therapeutics in. Sensitivity versus specificity find, read and cite all the research you need on researchgate. Bioanalysis of sirna and oligonucleotide therapeutics in. Many bioanalytical methods for oligonucleotides have been developed to. The importance of the length andor structure of fluorescently labeled. Request pdf on nov 1, 20, laixin wang and others published regulated bioanalysis of oligonucleotide therapeutics and biomarkers. If you are a society or association member and require assistance with obtaining online access instructions please contact our. The oligonucleotides and nucleic acid interest group meeting for asms 2017 was held on. Advances in quantitative bioanalysis of oligonucleotide. A ntisense oligonucleotides, sirnas, mirnas, aptamers, and other small.

An 18mer phosphorothioate oligonucleotide tl0901 was used as a model compound to demonstrate the assay specificity and sensitivity. Laixin wang vice president of bioanalytical at novabioassays and. Technical advances and demands for highthroughput accurate quantification of oligonucleotide therapeutics and biomarkers in pharmaceutical research and clinical diagnosis have aided evolution in quantitative bioanalysis of oligonucleotides. Recent advances in bioanalysis and metabolite profiling of oligonucleotide therapeutics 2019. In addition to the low assay specificity, the reported limit of the quantification of a competitive hybridization assay is 900 pm 5 ngml in plasma for a 15mer phosphodiester oligonucleotide 6. Laixin wang, sherry liu, qiuying zhu, scott reuschel and min meng tandem labs. Each method has been used for different study purposes for a period of time. Strategies for bioanalysis of an oligonucleotide class. Recent advances in bioanalysis and metabolite profiling of oligonucleotide therapeutics. Strategies for bioanalysis of an oligonucleotide class macromolecule from rat plasma using liquid chromatography tandem mass spectrometry. Tandem labs, 1121 east 3900 south, building c, suite 105, salt lake city, ut 84124, usa.

Provides an overview of the current stage in oligonucleotide therapeutics. Recent advances in bioanalysis and metabolite profiling of. Regulated bioanalysis of oligonucleotide therapeutics and. If the address matches an existing account you will receive an email with instructions to reset your password. Quantitatitive analysis of phosphorothioate oligonucleotide in.

Regulated bioanalysis of oligonucleotide therapeutics and biomarkers. Pdf this article summarizes bioanalytical avenues for the determination of. Samuel wainhaus associate director of bioanalytical at alnylam pharmaceuticals nucleic acidbased therapeutics. Pdf quantitatitive analysis of phosphorothioate oligonucleotide in. Importance of probe design for bioanalysis of oligonucleotides using. Hydrolysis of phase ii conjugates for lcms bioanalysis of. Use of lcmsms assays for macromolecules, like oligonucleotides, is challenging due to lack of sensitivity and low analyte recovery. Bioanalysis of sirna and oligonucleotide therapeutics in biological fluids and tissues. Laixin wang, sherry liu, qiuying zhu, scott reuschel and min meng.